DNA polymerase and carbohydrate metabolizing enzyme content of normal and leukemic glass column separated leukocytes.

S TUDIES OF THE enzyme content of leukocytes reported in the past’9 have been performed on mixed cell suspensions. Some attempts to estimate the enzyme values for individual cell types have been made2, 3. 8 on the basis of complex calculations after multiple assays of mixed cell suspensions with varied cell differential counts. The technic of separation of leukocytes on glass bead 12 now permits a more direct approach to this problem. The present study reports the results of assays of DNA polymerase and of five representative carbohydrate metabolizing enzymes ( glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, lactic dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase and isocitric dehydrogenase) performed on normal and leukemic leukocytes which were separated on glass columns.